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| Registros recuperados: 10 | |
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| Miura, Takayuki; Lhomme, Sebastien; Le Saux, Jean-claude; Le Mehaute, Philippe; Guillois, Yvonnick; Couturier, Elizabeth; Izopet, Jacques; Abranavel, Florence; Le Guyader, Soizick. |
| A hepatitis E outbreak, which occurred on a small isolated island, provided an opportunity to evaluate the association between the number of hepatitis E cases in the community and the concentration of virus detected in sewage. Samples were collected from the different sewage treatment plants from the island and analyzed for the presence of hepatitis E (HEV) virus using real-time RT-PCR. We demonstrated that if 1–4 % of inhabitants connected to a WWTP were infected with HEV, raw sewage contained HEV at detectable levels. The finding that such a small number of infected people can contaminate municipal sewage works raises the potential of the further distribution of the virus. Indeed, investigating the routes of transmission of HEV, including the potential... |
| Tipo: Text |
Palavras-chave: Hepatitis E virus; Wastewater treatment plant; Quantification. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00334/44482/44199.pdf |
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| van der Poel,W.H.M.; Pavio,N.; van der Goot,J.; van Es,M.; Martin,M.; Engel,B.. |
| Hepatitis E virus (HEV) is classified within the family Hepeviridae, genus Hepevirus. HEV genotype 3 (Gt3) infections are endemic in pigs in Western Europe and in North and South America and cause zoonotic infections in humans. Several serological assays to detect HEV antibodies in pigs have been developed, at first mainly based on HEV genotype 1 (Gt1) antigens. To develop a sensitive HEV Gt3 ELISA, a recombinant baculovirus expression product of HEV Gt3 open reading frame-2 was produced and coated onto polystyrene ELISA plates. After incubation of porcine sera, bound HEV antibodies were detected with anti-porcine anti-IgG and anti-IgM conjugates. For primary estimation of sensitivity and specificity of the assay, sets of sera were used from pigs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis E virus; Zoonosis; Swine; ELISA. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400334 |
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| Passos-Castilho,Ana Maria; de Sena,Anne; Domingues,Ana Lucia Coutinho; Lopes-Neto,Edmundo Pessoa; Medeiros,Tibério Batista; Granato,Celso Francisco Hernandez; Ferraz,Maria Lúcia. |
| Abstract Background Hepatitis E virus (HEV) can cause chronic infection with rapid progression to liver cirrhosis in immunocompromised patients. HEV seroprevalence in patients with Schistosoma mansoni in Brazil is unknown. We evaluated the prevalence of past or present HEV infection in schistosomiasis patients in Recife, Pernambuco, Brazil. A total of 80 patients with Schistosoma mansoni were consecutively enrolled in a cross-sectional study. Serum samples were tested for the presence of anti-HEV IgG antibodies by enzyme immunoassay (Wantai anti-HEV IgG, Beijing, China) and for the presence of HEV RNA using real time reverse transcriptase-polymerase chain reaction with primers targeting the HEV ORF2 and ORF3. Clinical and laboratory tests as well as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazil; Epidemiology; Hepatitis E virus; Schistosomiasis; Seroprevalence. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300007 |
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| Feurer, C.; Le Roux, A.; Rossel, R.; Barnaud, E.; Dumarest, M.; Garry, Pascal; Pavio, N.. |
| Pork ham muscle can be contaminated with HEV via blood vessels during viremia and represents a possible source of human contamination via the consumption of dried ham. This study evaluated the prevalence of HEV RNA in pork ham muscles and pork livers at slaughterhouses. Serology was determined on the corresponding serum samples. The apparent individual seroprevalence rate in the 49 pig farms studied was 59% [55.5%–61.4%]. None of the 1134 ham muscles tested was positive for the presence of HEV. HEV prevalence in paired liver samples was 2.8% with a level of contamination of up to 1.46 108 copies/g. Sequences of viral strains isolated from positive livers belonged to genotype 3 and subtypes 3c, 3e, 3f and 3j. Our results confirmed that raw pork liver food... |
| Tipo: Text |
Palavras-chave: Pork muscles; Hepatitis E virus; Food safety. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00405/51647/52196.pdf |
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| Vasconcelos,J.; Soliman,M.C.; Staggemeier,R.; Heinzelmann,L.; Weidlich,L.; Cimirro,R.; Esteves,P.A.; Silva,A.D.; Spilki,F.R.. |
| Hepatitis E virus (HEV) is highly disseminated among swine herds worldwide. HEV is also a threat to public health, since particularly genotypes 3 and 4 may cause acute hepatitis in human beings. No previous studies were done on the occurrence of HEV in environmental samples in Rio Grande do Sul, Brazil. In the present study, reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect the presence of HEV in swine feces and in effluents from slurry lagoons in farms located in the municipality of Teutônia, inside the area of swine husbandry in the state. Pooled fecal samples from the floor of pig barns from 9 wean-to-finish farms and liquid manure samples were collected from the slurry lagoons from 8 of these farms. From the pooled fecal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis E virus; Swine HEV; Slurry; Zoonosis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300777 |
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| Bricks,Guilherme; Senise,Jorge Figueiredo; Pott-Jr,Henrique; Grandi,Giuliano; Carnaúba-Jr,Dimas; Moraes,Hamilton Antonio Bonilha de; Granato,Celso Franscisco Hernandes; Castelo,Adauto. |
| ABSTRACT Background: Hepatitis E virus (HEV) infection in patients with pre-existing liver disease has shown high morbidity and lethality. The consequences of HEV superinfection in patients with chronic hepatitis C virus (HCV) infection are not fully understood. This study aimed to evaluate the association between the presence of anti-HEV antibodies, liver cirrhosis, and insulin resistance. Methods: A total of 618 patients chronically infected with HCV were included from three reference centers for viral hepatitis in São Paulo, Brazil. Presence of anti-HEV IgG was assessed by enzyme-linked immunosorbent assay (WANTAI HEV-IgG ELISA). Results: The seroprevalence of anti-HEV in patients with cirrhosis was significantly higher than in patients without... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis E virus; Hepatitis C virus; Liver cirrhosis; Insulin resistance. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100045 |
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| Bricks,Guilherme; Senise,Jorge Figueiredo; Pott Junior,Henrique; Grandi,Giuliano; Passarini,Amanda; Caldeira,Débora Bellini; Carnaúba Junior,Dimas; Moraes,Hamilton Antonio Bonilha de; Granato,Celso Franscisco Hernandes; Castelo,Adauto. |
| ABSTRACT Background and aims: Hepatitis E virus infection in patients with underlying chronic liver disease is associated with liver decompensation and increased lethality. The seroprevalence of hepatitis E virus in patients with chronic hepatitis C in Brazil is unknown. This study aims to estimate the seroprevalence of hepatitis E virus in patients with chronic hepatitis C and to describe associated risk factors. Methods: A total of 618 patients chronically infected with hepatitis C virus from three reference centers of São Paulo, Brazil were included. Presence of anti-HEV IgG was assessed by enzyme-linked immunosorbent assay (WANTAI HEV-IgG ELISA). Results: Out of the 618 patients tested, 10.2% turned out positive for anti-HEV IgG (95% CI 8.0–12.8%).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis E virus; Hepatitis C virus; Brazil; Seroprevalence. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200085 |
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| Registros recuperados: 10 | |
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